CRISPR-cas9 energy models for gRNA efficiency prediction¶
uCRISPR¶
This tool can predict on-target activity given 23bp gRNA.
https://github.com/Vfold-RNA/uCRISPR
Input¶
AGTCGT(23bp with PAM)
Usage¶
module load gcc/5.4.0
export DATAPATH=/home/yli11/HemTools/share/script/RNAstructure/data_tables
uCRISPR -on input.list > output
DNA_melting_bubbles_TIDD¶
This tool can predict destabilization score for each bp from the input sequence. The higher the score, the higher instability.
https://github.com/JanZrimec/DNA_melting_bubbles_TIDD
Input¶
any sequences > 10bp
Usage¶
module load conda3
source activate /home/yli11/.conda/envs/py2
module load matlab
module load java
DNA_melting -s "AGTAGTAGTAGTAGATAGTA"