CRISPR tools

• Crispresso2 for Base editor
• Base editor screening for gene functions
• cas9ENG
• Find number of off-targets
• Analysis pipeline for change-seq randomized assay
• Calculate custom defined editing frequency from Crispresso2 output
• Quantify base editing efficiency for crispressoPooled experiments
• Quantify prime editor off-target activity for crispressoPooled experiments
• Quantify base editing efficiency/cas9 indel frequency for Hybrid-Capture assay
• Crispresso2 for Prime Editor
• convert gRNA bed file to cutsite bed file
• Easy-Prime: pegRNA design
• CRISPR-cas9 energy models for gRNA efficiency prediction
• QC for gRNA quality, gRNA sequencing data
• Convert MaGeCK RRA sgRNA results to bw tracks
• Long-read sequencing to quantify large indels
• sgRNA design for disrupting TFBS
• Off-targets and Variants overlap

code @ github.