Transcript-level abundance quantification¶
module load kallisto/0.43.1
Note¶
Based on my own testing, a multi-mapped read will only be counted once in the abundance.tsv, but will occur multiple times in the pseudobam file.
If Kallisto multi-mapping reads, then one was selected at random. http://genomespot.blogspot.com/2015/08/how-accurate-is-kallisto.html
https://github.com/pachterlab/kallistobustools/issues/15