Target-Seq analysis¶
Summary¶
Input¶
Usage¶
Go to your data directory and type the following.
Step 0: Load python version 2.7.13.
module load python/2.7.13
Step 1: Run the program
idr_peaks.py -r1 R1_input -r2 R2_input -g hg19 --macs_genome hs
Note that if you are working on mouse genome, you have to change both -g and --macs_genome options, for example:
idr_peaks.py -r1 R1_input -r2 R2_input -g mm9 --macs_genome mm
Output¶
IDR peaks is shown in idr_peaks.bed
You can also find outputs from homer analysis: homer_motifs_result and idr_peaks.annotated.tsv
Ref: https://hbctraining.github.io/Intro-to-ChIPseq/lessons/07_handling-replicates-idr.html